Biological nanoparticles such as extracellular vesicles (EVs),
exosomes, viruses, or virus-like particles are in the growing interest
of the biosciences and nanomedicine. With the NTA technology, particles
are individually measurable in a physiological buffer and you observe
what you measure in a video. A new step forward in convenience and speed
for fluorescence studies of EV-antibody conjugates has been achieved
with the ZetaView® QUATT Lasers and fluorescence filters are
pre-selectable via the software. This procedure saves time and sample.
Typical application
Determining
the absolute concentration is one of the strengths of the NTA
technique. In scatter mode, the concentration of all particles is
measured. Changing subsequently to the fluorescence mode, marked
biologic nanoparticles are tracked. By using four lasers within a SOP
assisted sequence, it is possible to differentiate between four
different fluorescence dyes in the same sample. Phenotyping of
tetraspanins for instance, on vesicles and virus is facilitated,
positive and isotype control as well. To prepare instrument settings,
standard EVs may be used (HBM HansaBioMed).
Hong Kong and Macao customers only!
Please register as a A&P member to see the documents!