Biological nanoparticles such as extracellular vesicles (EVs),
exosomes, viruses, or virus-like particles are a rapidly growing area of
research in the life sciences and nanomedical field. The rapid in vitro
measurement of multiple physical parameters such as size,
concentration, surface charge, and phenotype characteristics is the
special feature of the Particle Metrix ZetaView®.
Nanoparticle
Tracking Analysis (NTA) captures the Brownian motion of each particle in
the video. Based on the different diffusion movements of large and
small particles in the surrounding liquid, the hydrodynamic diameter of
the particles is determined. Furthermore, the charge state of the
particle surface (zeta potential) can be measured via the movement of
the particles in an applied electric field.
Pattern parameters,
such as intensity fluctuations, surface geometry and shape of the
particles as well as particle concentration are documented at each
recording and can be used to distinguish sub-populations.
All these analyzes are carried out quickly and statistically reliably as required in the scattered light or fluorescence mode.
Antibody-conjugated EVs can be differentiated from membrane-enveloped vesicles labeled with corresponding intercalating dyes.
Depending on the type of sample and the measuring mode, the measuring range is between 15 nm and 5 μm.
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